Abstract: In order to establish an ideal research platform to make further study of the relationship between the two genes , hOGG1 and hMTH1, two cell models with gene defect were established. METHODS: The lentivirus vector with shRNA targeting human 8-oxoguanine DNA glycosylase 1(hOGG1) mRNA and 8-oxoguanine nucleoside triphosphatase 1(hMTH1) mRNA were transfected into 293FT cells by liposome. Then the lentivirus supernatant was obtained and used for infecting human embryonic lung fibroblasts(HFL). The defective cells were selected with blasticidin, and fluorescence microscope was used as a tool for observing the packaging efficiency and infection efficiency. The efficacy of gene knockout was tested by Real-time RT PCR. RESULTS: The lentivirus with high titer were obtained and the hOGG1- deficient and the hMTH1- deficient HFL cell strains were obtained after blasticidin selection. The level of hOGG1 and hMTH1 mRNA expression were decreased, 90% and 60%, respectively. CONCLUSION: Lentivirus packaging technology could be mastered skillfully. hOGG1-deficient and hMTH1-deficient HFL cell models were successfully established.

Key words: lentivirus, RNAi, hOGG1, hMTH1